We are developing novel coherent spectroscopic and microscopy methods to follow biochemical reactions across multiple time and energy scales. Our primary tool is two-dimensional photon echo spectroscopy, which provides a correlation map that links dipoles or oscillators during two different periods in time. These maps provide high-resolution snapshots of the electronic or vibrational structure of the molecule as it undergoes some dynamical process such as energy or electron transfer, bond breaking and forming, or structural rearrangement. Critical to our success in elucidating the molecular mechanisms of complex biochemical processes are the use of complementary techniques which can probe both ensemble and single-molecule properties.