Welcome to Milan Mrksich's Group and the
Laboratory for BioInterface Science and Engineering
My group’s interests overlap
chemistry, biology and engineering, with an emphasis on the design and
synthesis of materials that are biologically active and in applications
of the materials to relevant problems in the biological and medical
sciences. Much of our work uses self-assembled monolayers of
alkanethiolates on gold to prepare model surfaces that are structurally
defined, yet that can have complex compositions and present the
ligands in spatially-organized patterns. We pioneered the design of
‘dynamic substrates’ that present ligands whose activities can be
switched on and off in response to electrical or optical signals,
particularly for studies that address the responses of adherent cells
to changes in the extracellular matrix. These mimics of the
extracellular matrix have led the way to the discovery of novel ligands
that mediate cell adhesion. We have also developed robust surface
chemistries for preparing biochip arrays and that are compatible with
new analytical methods for analyzing the arrays. For example, we have
developed the SAMDI method, which uses mass spectrometry to analyze
the arrays, and we have extended this method to the first label-free
approach for high throughput screening, to the functional annotation of
recently sequenced genes and towards an understanding of the networks
that regulate protein acetylation. Finally, a recent program is
creating defined systems for exploring biochemical reactions to
understand the role that localization of enzymes and substrates play in
controlling reaction networks.
Congratulations to Elamar, Sarah and Adam for successfully completing their qualification exams to enter Ph.D. candidacy!
Congratulations to Kazi, Azmain and Eric for having their paper, " Traceless Immobilization of Analytes for High-Throughput Experiments with SAMDI Mass Spectrometry " accepted in JACS!
Congratulations to Jen, Justin, Juliet and Paul for having their paper, "An Immobilized Enzyme Reactor for Spatiotemporal Control over Reaction Products" accepted in Small!
Congratulations to Shuheng for the acceptance of her paper, "An Unusual Salt Effect in an Interfacial Nucleophilic Substitution Reaction" in Langmuir!
Congratulations to Maria Cabezas on successfully defending her thesis!
Congratulations to Sherry, Hsin-Yu and our collaborators in the Ameer group for acceptance of their paper "A Potent Laminin-Inspired Antioxidant Regenerative Dressing Accelerates Wound Healing in Diabetes in Proc. Natl. Acad. Sci.!
Congratulations to Justin and Yao, for having their paper "Synthesis of Cyclic Megamolecules" accepted in JACS!
Congratulations to Kevin Metcalf for being awarded an American Cancer Society Postdoctoral Fellowship!
Congratulations to Blaise Kimmel for being awarded an NSF Graduate Research Fellowship!
Congratulations to Liang and our collaborators in the Jewett lab for the acceptance of our paper “Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases” in Nature Chemical Biology!.
Congratulations to Lindsey and Hsin-Yu, for having their review “Peptide Arrays: Development and Application” accepted in Analytical Chemistry.
Congratulations to Caleb and Mike and our collaborators in the Fierke group, for having their paper “Active Site Metal Identity Alters HDAC8 Substrate Selectivity: A Potential Novel Regulatory Mechanism” accepted in Biochemistry.
Congratulations to Sarah, Jeffrey and our collaborators in the Liedberg group, for having their paper “A Bottom-Up Proteomic Approach to Identify Substrate Specificity of Outer Membrane Protease OmpT” accepted in Angew Chem.
Congratulations to Lindsey and our collaborators Albert and Neda Bagheri, for having their paper “Machine Learning on SAMDI Mass Spectrometry Signal to Noise Ratio Improves Peptide Array Designs” accepted in Analytical Chemistry.
Congratulations to Patrick, for having his paper “An Assay Based on SAMDI Mass Spectrometry for Profiling Protein Interaction Domains” accepted in JACS.
Megan Burton, undergrad in the Mrksich group, was awarded the Fulbright scholarship or the 2017-2018 year. She will be working with Dr. Raphaeil Margueron at the Curie Institute in Paris, comparing the genetic modifications of different cancerous tumors.
Congratulations to Maria for having her paper "Nanopatterned Extracellular Matrices Enable Cell-Based Assays with a Mass Spectrometric Readout" accepted in NanoLetters.
Congratulations to Pradeep and our collaborators in the Van Duyne and Schatz Groups, for having their paper “Bisboronic Acids for Selective, Physiologically Relevant Direct Glucose Sensing with Surface-Enhanced Raman Spectroscopy” accepted in JACS.
Milan discusses the emerging field of Synthetic Biology and its rapidly developing impact on cancer research. Link to article here.
Congratulations to Eric, Pradeep and our collaborators, Professor Al George and Lyndsey Anderson, for having their paper “Measuring Drug Metabolism Kinetics and Drug-Drug Interactions with SAMDI Mass Spectrometry” accepted in Analytical Chemistry.
Congratulations to Justin and our collaborators in the Hupp and Farha groups for having their paper “Toward Design Rules for Enzyme Immobilization in Hierarchical Mesoporous Metal-Organic Frameworks” accepted in CHEM.
Congratulations to Eric and Maria for their paper “Cellular Assays with a Molecular Endpoint Measured by SAMDI Mass Spectrometry” accepted for publication in Small.
Milan was interviewed for a story on the role of big data in biology. You can find the article here.
Milan, Director of the Center for Synthetic Biology, invites you to celebrate the Center's launch event on March 22, 3pm-6pm in the Ford ITW Calssroom, Evanston campus.
Milan is presenting a talk on the use of SAMDI to measure enzyme activities in lysates from single cells, at this week's Pittcon meeting.
Congratulations to Kristin on acceptance of her paper "Micropatterning Facilitates the Long-Term Growth and Analysis of iPSC-Derived Individual Human Neurons and Neuronal Networks" by Advanced Healthcare Materials.
Milan and Senator Dick Durbin discuss the increase in NIH's budget
Nanopatterned Extracellular Matrices Enable Cell-Based Assays with a Mass Spectrometric Readout. Cabezas, M., Mirkin, C.M. and Mrksich, M. Nano Lett., 2017, 17, 1373-1377. [PDF]
An Assay Based on SAMDI Mass Spectrometry for Profiling Protein Interaction Domains. O’Kane, P. and Mrksich, M. J. Am. Chem. Soc., 2017, 139, 10320-10327. [PDF]
Machine learning on Signal to Noise Ratio Improves Peptide Array Design in SAMDI Mass Spectrometry. Xu, A., Szymczak, L., Mrksich, M. and Bagheri, N. Anal. Chem., 2017, 89, in press. [PDF]
A Bottom-Up Proteomic Approach to Identify Substrate Specificity of Outer Membrane Protease OmpT. Wood, S.E., Sinsinbar, G.,Gudlur, S., Nallani, M., Huang, C.-F., Liedberg, B. and Mrksich, M. Ang. Chem., 2017, 56, in press. [PDF]
Active Site Metal Identity Alters Histone Deacetylase 8 Substrate Selectivity: A Potential Novel Regulatory Mechanism. Castaneda, C.A., Lopez, J.E., Joseph, C.G., Scholle, M.D., Mrksich, M. and Fierke, C.A., Biochemistry, 2017, 56, in press. [PDF]
Peptide Arrays: Development and Application. Szymczak, L.C., Kuo, H.-Y. and Mrksich, M., Anal. Chem., 2018, 90, 266-282. [PDF]
Efficient Syntheses of Diverse, Medicinally Relevant Targets Planned by Computer and Executed in the Laboratory. Klucznik, R., Mikulak, B., McCormack, M.P., Lima, H., Szymkuc, S., Bhowmick, M., Molga, K., Zhou, Y., Rickershauser, L., Gajewska, E.P., Toutchkine, A., Dittwald, P., Startek, M.P., Kirkovits, G.J., Roszak, R., Adamski, A., Sieredzinska, B., Mrksich, M., Trice, S.L.J. and Grzybowski, B.A. Chem., 2018, in press. [PDF]
Design of Glycosylation Sites by Rapid Synthesis and Analysis of Glycosyltransferases. Kightlinger, W., Lin, L., Rosztoczy, M., Li, W., DeLisa, M.P., Mrksich, M. and Jewett, M.C., Nat. Chem. Biol., 2018, in press.
Synthesis of Cyclic Megamolecules. Modica, J.A., Lin, Y. and Mrksich, M. J. Am. Chem. Soc. 2018, 140, in press.
For general inquiries contact MrksichGroup at gmail.com
2145 Sheridan Road
B490 Technolgical Institute
Evanston IL 60208